Detection of the ADAM proteins in the ejaculated bovine sperm

The current study aimed to detect specific types of ADAM proteins in the bovine sperm. Histology tissue male reproduction was involved with testis, head, body, and tail epididymis. Results showed that testis composed parenchyma contained seminiferous tubules which are surrounded by two layers externally tunica vaginalis internally albuginea. PAS positive lumen, epithelium body epididymis, Whereas Alcian blue very low intensity stains sections contents Fresh ejaculated sperms were collected separated from seminal plasma, sperm extracted run on an electrophoresis gel. Then, bands have been stained coomassie stain, after that, pieces cut gels amino acids digestion proteins, uploaded a mass spectrometry machine. Our data profiled analysed detected characterised included: ADAM1, ADAM2, ADAM3A, ADAM10 ADAM32, uncharacterized ADAM1B, ADAM7, ADAM9, ADAM12, ADAM15, ADAM17.